ANTIBODIES - Purification

The monoclonal antibody purification and polyclonal serum services include different steps and proceedings according to the origin of the sample.

Purification by affinity with protein A or G in rabbit:

- Polyclonal serums.

- Monoclonal antibodies.

- Ascitic fluid.

- Cultivation supernatant.

Purification protocol

	DAY	0	4	6
Protein A or G	Precipitation with ammonium sulphate	x
	Purification		x

Ref.	Product
SE-4774	Purification by protein A or G in rabbit (<10 mg)
SE-4848	Purification by protein A or G in rabbit (>10 mg)

Purification by affinity with antigens (peptides or proteins) in rabbit:

- Polyclonal serum.

- Monoclonal antibodies.

Purification protocol

	DAY	0	4	6
Purification by affinity with antigens	Column coated with the antigen		x
	Purification			x

In last case, the purification system will depend on the use that the client will make of the antibody. If you need a different purification system, contact us.

The price of the service is established based on the number of mg requested by the client.

Ref.	Product
SE-4777	Purification by affinity in rabbit (<10 mg)
SE-4778	Purification by affinity in rabbit (>10 mg)

Purification in other animals

Ref.	Product
SE-4830	Purification by affinity with protein A in guinea pig
SE-4834	Purification by affinity with antigens in guinea pig
SE-4831	Purification by affinity with protein A in goat
SE-4835	Purification by affinity with antigens in goat
SE-4832	Purification by affinity with protein G in rat
SE-4836	Purification by affinity with antigens in rat
SE-4833	Purification by affinity with protein A in chicken
SE-4837	Purification by affinity with antigens in chicken
SE-5245	Purification with antigens in hen

Antigenicity analysis of Protein Sequences:

Biomedal offers to its clients a Protein Analysis service which includes the selection of the most antigenic peptidic sequence, the homogeneity study according to the protein in living beings, the possible identification of the signal peptide, of the transmembrane domains and other structural motives.

Our services include:

1. Identification of specific antigenic peptides. We can analyze the protein sequence totally or partially. The parameters that we are analyzing are: antigenicity, hydrophobicity and accessibility. Our programmes find the regions with bigger antigenicity, hydrophobicity and accessibility (between 10 to 20 amino acids). This information will sent by e-mail or fax, using graphic means to ease understanding. The programmes used never guarantee success but allow us to get an idea of what is occurring, orienting toward the regions of better success probability.

2. Search homologies with others species. The sequences chosen are compared with those of diverse databases to discover possible homologies. In this process we are seeking the sequence or part of the sequence which is the least similar to the rest of the homologous sequences of other other species to avoid undesired crossed reactivity. In the last case the researcher will decide if the homology given by the protein is a problem or not. This information will be sent by e-mail or fax. The information will be verified extremely carefully to avoid undesired recognition of the antibody.

3. Analysis of the secondary and third structure. Biomedal can analize the possible secondary structure of the three or four sequences proposed which, along with the previous information, will help us to decide which peptidic sequence to keep.

4. Types of analysis. Our service offers, among others, the following analyses:

ANALYSIS

Protein identification and characterization.

DNA-Protein interaction.

AGADIR - An algorithm to predict the content of peptide helices.

APSSP - Peptide secondary structure prediction.

DLP - Prediction of RIKEN domains.

MITDISC - Search of mitochondrial sequences.

NUDISC - Localization of nuclear signals.

PSORT - Prediction of cellular localization.

TargetP - Prediction of sub-cellular localization.

Prediction of possible post-transcriptional modifications.

BLAST - Search homologies.