> Lytag-2-phase

LYTAG Two-Phase is a protein purification system based on the use of two aqueous components. The method relies on the affinity of the protein tag LYTAG for one of the two-phase components, allowing recombinant protein separation and purification from cellular extracts or culture media. In the procedure, the LYTAG-fused protein is retained in one of the aqueous phases while most of the undesired proteins can be removed by simply discarding the opposite phase. After replenishing the system with fresh phase, the protein of interest can be easily recovered in it, with high purity, by reversing its localization with the addition of choline, the specific LYTAG ligand.

This system is particularly well suited for industries and laboratory specialized in protein separation and purification, as it is simple, cost efficient, time saving and highly versatile for scaling up protein purification process, representing a convenient alternative to solid resins.

Applications:

• – Rapid and scalable purification of recombinant LYTAG fusion proteins.

Advantages:

• – Quick method. Separation can be completed within few minutes, minimizing the effects of proteases. It is specially convenient when large volumens (>10 ml) of cellular extracts or culture media need to be processed (usually requiring prolonged flow times when using chromatography columns).
• – Easy and inexpensive protein purification process.
• – No special equipment requirements.
• – It offers, like every aqueous two-phase systems, mild conditions in the separation of labile proteins. Purification can be easy and safely performed at low temperatures, requiring only a refrigerated centrifuge and an ice water bath.
• – Scalable method.
• – High purification efficiency (>95% purity), comparable to the use of solid matrixes.
• – Optimum performance in downstream fermentation processes.
• – Good alternative to conventional solid resins.

REF.	PRODUCT	FORMAT
KT-4697	LYTAG 2-PHASE Purification System	20 ml
KT-4739	LYTAG 2-PHASE Purification System	80 ml
RS-4700	Lower Solution (LYTAG 2-Phase Purification System)	20 ml
RS-4741	Lower Solution (LYTAG 2-Phase Purification System)	40 ml
RS-4701	Top Solution (LYTAG 2-Phase Purification System)	40 ml
RS-4740	Top Solution (LYTAG 2-Phase Purification System)	80 ml
RS-4742	2-Phase LYTAG Purification Solution Kit	20 ml
RS-4743	2-Phase LYTAG Purification Solution Kit	80 ml
RS-4702	LYT 2-Phase Wash (2x) (LYTAG 2-Phase Purification System)	125 ml
RS-4703	Choline Chloride 3M (LYTAG 2-Phase Purification System)	12 ml